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Die bekanntesten Nitrifikanten sind Nitrobacter, eine Gattung nitratbildender Bakterien und Nitrosomonas, eine Gattung nitritbildender Bakterien.
Nitrobacter und Nitrosomonas, die im Boden und im Wasser leben, ernähren sich autotroph unter Ausnutzung von Energie, die durch die Nitrifikation gewonnen wird. Bedingt durch ihre geringen Wachstumsraten ist es notwendig, besondere Milieubedingungen zu gewährleisten, um diese Bakterien im Wasserbereich besonders effektiv einsetzen zu können.