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Bezeichnung für die Lebensweise von Organismen, die zum Leben keinen freien Sauerstoff benötigen und für eine chemische Reaktionsweise, die unter Ausschluss von Sauerstoff abläuft.
Die anaerobe Reinigung von Wasser erfolgt unter anderem in Biofiltern und im Boden (sofern ein Bodenfluter vorhanden ist) und reinigt dort insbesondere stark organisch verschmutztes Wasser.
Liegt hingegen zumindest ungelöst gebundener Sauerstoff vor, handelt es sich um ein anoxisches Milieu. Liegt gelöster Sauerstoff vor, ist das Milieu aerob.